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PCR Sequencing

Our Mutation Analysis service includes PCR optimization, amplicon production and purification.  Whether confirming that a mutation has occurred or precisely determining the genotype and specific allelic event, leveraging our expertise ensures that your genome editing functions efficiently.

Services Include:

Extraction of genomic DNA from single-isolate cell samples

Amplicon selection and primer design for on- and off-target regions

Assay development and optimization

PCR amplification of genomic DNA

High-throughput PCR purification and bi-directional DNA sequencing

Automated and manual sequencing results analysis

Report identifying nucleotide-level mutations

Translated amino acid calls available upon request